Which chromatography system uses a stationary phase that is liquid absorbed on particles packed in a column and a liquid moving phase pumped through a column?

Which chromatography system uses a stationary phase that is liquid absorbed on particles packed in a column and a liquid moving phase pumped through a column?

• A. Thin-layer
• B. High-performance liquid
• C. Ion-exchange
• D. Gas-liquid

Answer: (B)

When the stationary phase is a liquid film absorbed on solid particles packed inside a column, and the moving phase is a liquid forced through the column, you’re looking at high-performance liquid chromatography. This setup uses a liquid stationary phase bonded or absorbed onto a solid support (like silica beads) and a liquid mobile phase pumped through under high pressure, which gives high-resolution separations with sharp peaks. This differs from thin-layer chromatography, where the stationary phase is a solid coating on a plate and the solvent moves by capillary action without a column. Gas-liquid chromatography uses a gas as the mobile phase with a liquid stationary phase, typically in a capillary or packed column. Ion-exchange chromatography relies on ionic interactions on a solid resin with functional groups, not a liquid film on a packed column.

When the stationary phase is a liquid film absorbed on solid particles packed inside a column, and the moving phase is a liquid forced through the column, you’re looking at high-performance liquid chromatography. This setup uses a liquid stationary phase bonded or absorbed onto a solid support (like silica beads) and a liquid mobile phase pumped through under high pressure, which gives high-resolution separations with sharp peaks.

This differs from thin-layer chromatography, where the stationary phase is a solid coating on a plate and the solvent moves by capillary action without a column. Gas-liquid chromatography uses a gas as the mobile phase with a liquid stationary phase, typically in a capillary or packed column. Ion-exchange chromatography relies on ionic interactions on a solid resin with functional groups, not a liquid film on a packed column.