At the isoelectric point, a protein has what net charge?

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Multiple Choice

At the isoelectric point, a protein has what net charge?

Explanation:
At the isoelectric point, the protein has no net charge. This occurs because the pH is such that the positive charges from protonated amino groups balance the negative charges from deprotonated carboxyl groups and other ionizable groups. With this balance, the overall charge sums to zero. If the pH drops below the isoelectric point, more groups become protonated, giving a net positive charge; if the pH rises above it, more groups lose protons, giving a net negative charge. So the defining result at the isoelectric point is a net zero charge.

At the isoelectric point, the protein has no net charge. This occurs because the pH is such that the positive charges from protonated amino groups balance the negative charges from deprotonated carboxyl groups and other ionizable groups. With this balance, the overall charge sums to zero. If the pH drops below the isoelectric point, more groups become protonated, giving a net positive charge; if the pH rises above it, more groups lose protons, giving a net negative charge. So the defining result at the isoelectric point is a net zero charge.

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